Adaptive Prefetching for Device-Independent File I/O

Device independent I/O has been a holy grail to operating system designers since the early days of UNIX. Unfortunately, existing operating systems fall short of this goal for multimedia applications. Techniques such as caching and sequential read-ahead can help mask I/O latency in some cases, but in others they increase latency and add substantial jitter. Multimedia applications, such as video players, are sensitive to vagaries in performance since I/O latency and jitter affect the quality of presentation. Our solution uses adaptive prefetching to reduce both latency and jitter. Applications submit file access plans to the prefetcher, which then generates I/O requests to the operating system and manages the buffer cache to isolate the application from variations in device performance. Our experiments show device independence can be achieved: an MPEG video player sees the same latency when reading from a local disk or an NFS server. Moreover, our approach reduces jitter substantially

Underwater communications system for use by free swimming divers.

http://archive.org/details/underwatercommun00ste

Fine-grain Period Adaptation in Soft Real-Time Environments

Reservation-based scheduling delivers a proportion of the CPU to jobs over a period of time. In this paper we argue that automatically determining and assigning this period is both possible and useful in general purpose soft real-time environments such as personal computers and information appliances. The goal of period adaptation is to select the period over which a job is guaranteed to receive its portion of the CPU dynamically and automatically. The choice of period represents a trade-off between the amount of jitter observed by the job and the overall efficiency of the system. Secondary effects of period include quantization error, job priority, changes in memory behavior, and battery life of portable devices. In addition to discussing these issues in detail, we present the design and evaluation of a mechanism for period adaptation based on feedback control. Together with an existing proportion allocation mechanism, this period adapter merges the benefits of best-effort and reservation-based systems by providing the fine-grain control of reservation-based scheduling without requiring applications to specify their own resource needs in advance

Location Independent Names for Nomadic Computers

Recent advances in the Domain Name System (DNS) and the Dynamic Host Configuration Protocol (DHCP) have enabled a new approach to supporting mobile users: location independent naming. In this approach, machines use the same hostname from any internet location, but use an IP address that corresponds to their current location. We describe a protocol that implements location independent naming for nomadic computers, i.e., machines that do not need transparent mobility. Our protocol allows hosts to move across security domains, uses existing protocols, and preserves existing trust relationships. Therefore, it preserves the performance and security of normal IP for nomadic computers at the expense of not providing the transparent mobility of Mobile IP. We contend that this is a reasonable trade-off for nomadic computing

Using Dynamic Optimization for Control of Real Rate CPU Resource Management Applications

In this paper we design a proportional-period optimal controller for allocating CPU to real rate multimedia applications on a general-purpose computer system. We model this computer system problem in to state space form. We design a controller based on dynamic optimization LQR tracking techniques to minimize short term and long term time deviation from the current time stamp and also CPU usage. Preliminary results on an experimental set up are encouraging

The cost effectiveness of vaccinating against Lyme disease.

To determine the cost effectiveness of vaccinating against Lyme disease, we used a decision tree to examine the impact on society of six key components. The main measure of outcome was the cost per case averted. Assuming a 0.80 probability of diagnosing and treating early Lyme disease, a 0.005 probability of contracting Lyme disease, and a vaccination cost of $50 per year, the mean cost of vaccination per case averted was$4,466. When we increased the probability of contracting Lyme disease to 0.03 and the cost of vaccination to $100 per year, the mean net savings per case averted was$3,377. Since few communities have average annual incidences of Lyme disease >0. 005, economic benefits will be greatest when vaccination is used on the basis of individual risk, specifically, in persons whose probability of contracting Lyme disease is >0.01

Effect of HLA DR epitope de-immunization of Factor VIII \u3ci\u3ein vitro\u3c/i\u3e and \u3ci\u3ein vivo\u3c/i\u3e

T cell-dependent development of anti-Factor VIII (FVIII) antibodies that neutralize FVIII activity is a major obstacle to replacement therapy in hemophilia A. To create a less immunogenic therapeutic protein, recombinant FVIII can be modified to reduce HLA binding of epitopes based on predicted anchoring residues. Here, we used immunoinformatic tools to identify C2 domain HLA DR epitopes and predict site-specific mutations that reduce immunogenicity. Epitope peptides corresponding to original and modified sequences were validated in HLA binding assays and in immunizations of hemophilic E16 mice, DR3 and DR4 mice and DR3 × E16 mice. Consistent with immunoinformatic predictions, original epitopes are immunogenic. Immunization with selected modified sequences lowered immunogenicity for particular peptides and revealed residual immunogenicity of incompletely de-immunized modified peptides. The stepwise approach to reduce protein immunogenicity by epitope modification illustrated here is being used to design and produce a functional full-length modified FVIII for clinical use

Common and Unique Contributions of Decorin-Binding Proteins A and B to the Overall Virulence of Borrelia burgdorferi

As an extracellular bacterium, the Lyme disease spirochete Borrelia burgdorferi resides primarily in the extracellular matrix and connective tissues and between host cells during mammalian infection, where decorin and glycosaminoglycans are abundantly found, so its interactions with these host ligands potentially affect various aspects of infection. Decorin-binding proteins (Dbps) A and B, encoded by a 2-gene operon, are outer surface lipoproteins with similar molecular weights and share approximately 40% identity, and both bind decorin and glycosaminoglycans. To investigate how DbpA and DbpB contribute differently to the overall virulence of B. burgdorferi, a dbpAB mutant was modified to overproduce the adhesins. Overproduction of either DbpA or DbpB resulted in restoration of the infectivity of the mutant to the control level, measured by 50% infectious dose (ID50), indicating that the two virulence factors are interchangeable in this regard. Overproduction of DbpA also allowed the mutant to disseminate to some but not all distal tissues slightly slower than the control, but the mutant with DbpB overproduction showed severely impaired dissemination to all tissues that were analyzed. The mutant with DbpA overproduction colonized all tissues, albeit generating bacterial loads significantly lower than the control in heart and joint, while the mutant overproducing DbpB remained severely defective in heart colonization and registered bacterial loads substantially lower than the control in joint. Taken together, the study indicated that DbpA and DbpB play a similar role in contribution to infectivity as measured by ID50 value but contribute differently to dissemination and tissue colonization

Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph

Borrelia burgdorferi, the causative agent of Lyme disease, is transmitted to humans by bite of Ixodes scapularis ticks. The mechanisms by which the bacterium is transmitted from vector to host are poorly understood. In this study, we show that the F(ab)2 fragments of BBE31, a B.burgdorferi outer-surface lipoprotein, interfere with the migration of the spirochete from tick gut into the hemolymph during tick feeding. The decreased hemolymph infection results in lower salivary glands infection, and consequently attenuates mouse infection by tick-transmitted B. burgdorferi. Using a yeast surface display approach, a tick gut protein named TRE31 was identified to interact with BBE31. Silencing tre31 also decreased the B. burgdorferi burden in the tick hemolymph. Delineating the specific spirochete and arthropod ligands required for B. burgdorferi movement in the tick may lead to new strategies to interrupt the life cycle of the Lyme disease agent